Mobilization of Tn1721-like structure harboring blaCTX-M-27 between P1-like bacteriophage in Salmonella and plasmids in Escherichia coli in China

The aim of this study was to explore the characteristics blaCTX-M-27 carriage and mobilization in Salmonella Escherichia coli isolates from food-producing animals China. A total 2280 E. 229 collected food June 2003 September 2014 were screened for presence gene. blaCTX-M-27-positive typed plasmid DNA sequenced determine genetic context types present. Bacterial fitness evaluated by growth curve stability vitro. CTX-M-27-positive (18, 0.79 %) (34, 14.85 detected. PFGE profiles strains revealed a wide variety genotypes S. Indiana most prevalent serotype. Replicon typing, S1-PFGE hybridization CTX-M-27-carrying plasmids confirmed that gene located on IncFII (12/18), IncN (4/18), non-typeable (2/18) P1-like bacteriophage (21/34), IncP (4/34), IncFIB (2/34), IncHI2 IncA/C (1/34) Salmonella. Comparison analysis they shared same structure contained an identical with Tn1721-like ΔISEcp1B-blaCTX-M-27-IS903D-iroN-Δmap-Tn1721. In addition, tests indicated more stable than absence cefotaxime selective pressure. These results demonstrate transposons harboring CTX-M-27 could be mobilized between different disseminated among